Composite

Part:BBa_J64974:Design

Designed by: reid williams   Group: Voigt Lab   (2007-03-20)


small molecule controlled, riboregulator based XOR gate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 60
    Illegal XbaI site found at 441
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 649
    Illegal NheI site found at 612
    Illegal NheI site found at 635
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 60
    Illegal XbaI site found at 441
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 649
    Illegal XbaI site found at 60
    Illegal XbaI site found at 441
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The presence of either (but not both) of the keys unlocks a blocked RBS to allow translation of a CFP degradation tag fusion. The two keys have a greater affinity to each other than to the lock, so the presence of both does not unlock the blocked RBS. Still contains an EcoR1 site that needs to be fixed. Conceived by Laura Lavery.

Source

The Collins lab riboregulators and existing registry parts.

References